角质化细胞

来自 : 发布时间：2024-05-15

角质化细胞

  \"正常人表皮角质形成细胞（NHEK）少年包皮，单一捐献者” ，（NormalHumanEpidermalKeratinocytes(NHEK)juvenileforeskin,singledonor，500,000cryopreservedcells）。

   以下是产品的详细信息，可点击链接查看：
http://www.promocell.com/fileadmin/promocell/PDF/C-12001.pdf
http://www.promocell.com/fileadmin/promocell/MSDS/C-12001.pdf 
 
  正常的人类皮肤角质形成细胞（NHEK）分离自青少年包皮的表皮，以及成年人各个部位的皮肤，例如面部、乳房、腹部和大腿*。这些细胞是表皮中的主要细胞类型，占据了细胞总数的90%。
  表皮角质化细胞来自皮肤基底层，能穿过表皮各个层次上移。在这个移动过程中，这些细胞逐渐分化，并产生形态学改变，直到到达皮肤的角质层，在角质层，这些细胞形成一层无核的、扁平的和高度角质化的鳞状细胞。这层细胞形成一层有效的屏障，防止外来物质进入到机体，同时尽可能降低水分流失。
角质化细胞也能够产生一系列细胞因子、生长因子、白细胞介素和补体因子。因此，角质化细胞对伤口愈合、炎症和免疫应答都具有重要作用。
Thecellshavebeentested:Cytokeratinpositive
 
images/brand/Keratinocytes_2.jpg

 
应用范围

• 伤口愈合研究
• 皮肤发育和分化研究
• 皮肤病学研究
• 药物摄取研究
• 制药学测试
• 化妆品和毒理学检测
• 肿瘤学

特点

• 细胞角蛋白阳性
• 青少年包皮或成年人不同部位的皮肤上来源的也有供应
• 单一捐献者和混合捐献者来源的都有供应

推荐的培养基及试剂

• 角质细胞生长培养基2
• 细胞分离试剂盒
• Cryo-SFM

相关产品

• NHEK.f细胞团块
• NHEK.f混合来源的细胞团块
• NHEK成年人来源的细胞团块
• NHEK混合成年人来源的细胞团块
• 正常人类真皮成纤维细胞
• 正常人类表皮黑色素细胞
• PromoFectin转染试剂

 
以下是NHEK细胞的相关问题，可参考：
http://www.promocell.com/index.php?id=1049&tx_na15knowledgebase_pi1[categories][]=3&no_cache=1&tx_na15knowledgebase_pi1[sword]=NHEK
What\"sthedifferencebetweenNHEK.f(C-12001)andNHEK.fpooled(C-12005)?
NHEK.fareisolatedfromtissuesamples(foreskin)ofsingledonors,agedbetween1-10years. 
NHEK.fpooledarepreparedfromtheforeskinsof3individualdonors. ThecellsofeachdonorareexpandedinseparateTCvessels andthecellsarepooledaftersecondaryculture, beforecryopreservation.
BothNHEKfromsingledonorandNHEKpooledareinP2afterthawing.
DothePromoCellkeratinocytesneedfeedercells?
IfyouaregrowingourNHEKinPromoCellKeratinocyteGrowthMedium2(C-20011),youdon\"tneedanyfeedercells.Thecellswillgrowasamonolayerinconventionaltissuecultureflasks.
HowdoesPromoCelldeterminethephototypeofskintissuedonors?
Weusetheclassificationofskintypes(phototypeI-VI)accordingtoFitzpatrick.Thisisdeterminedbythepatient\"sskincolour(white,brownorblackskin),colorofeyesandhair,andbytheburning/tanningABIlity,i.e.bytheamountofmelaninpigmentintheskin.
I:Palewhiteskin,blue/hazeleyes,blond/redhair,alwaysburns,doesnottan
II:Fairskin,blueeyes,burnseasily,tanspoorly
III:Darkerwhiteskin,tansafterinitialburn
IV:Lightbrownskin,burnsminimally,tanseasily
V:Brownskin,rarelyburns,tansdarklyeasily
VI:Darkbrownorblackskin,neverburns,alwaystansdarkly 
DonorswithphototypeIandIIareclassifiedasL(lightlypigmented),withphototypeIIIandIVasM(moderatelypigmented),andwithphototypeVandVIasD(darklypigmented).
Informationonthephototypeisavailableformostourcelllotsisolatedfromjuvenileoradultskin.
IsitpossIBLetoobtainothercelltypes,e.g.keratinocytes,fromthesamefibroblastdonor?
Yes,inprincipleitispossibletoisolatekeratinocytes(NHEK),melanocytes(NHEM),dermalmicrovascularendothelialcells(HDMEC),andfibroblasts(NHDF)fromthesameskinsample.
Aswehaveastrictqualitycontrolsystem,somecellpreparationscanfailtopassQCandaren\"treleasedforsale.Therefore,pleasecontactourTechnicalCustomerServiceifyouneeddifferentcelltypesfromthesamedonorsothatwecancheckourinventory.
WhatisthetrypsinizationtimewithPromoCellkeratinocytes?
Thetrypsinizationtimeofprimarykeratinocytes(andepithelialcellsingeneral)isusuallylongerthanofothercells.Dependingonthecelllot,ittakesbetween5and10minatroomtemperature.Youcanacceleratethedetachmentbygentlytappingtheflaskassoonasthecellsshrinkandroundup.Alternatively,youcanuseaccutase(C-41310)at37°Cwhichhasbeenshowntonotaffectcellularviabilityevenatlongerincubationtimes.
 
参考文献——以下是近年来使用我们PromoCell的NHEK细胞进行试验研究，在国际知名杂志所发表的文章，如下：
Residualantimicrobialeffectofchlorhexidinedigluconateandoctenidinedihydrochlorideonreconstructedhumanepidermis
Muelleretal.;SkinPharmacolPhysiol.2014;27(1):1-8
Demonstrationofamelanoma-specificCD44alternativesplicingpatternthatremainsqualitativelystable,butshowsquantitativechangesduringtumourprogression
Raso-Barnettetal.;PLoSOne.2013;8(1):e53883
Arylhydrocarbonreceptorrepressor(AhRR)functionrevisited:repressionofCYP1activityinhumanskinfibroblastsisnotrelatedtoAhRRexpression
Tiggesetal.;JInvestDermatol.2013Jan;133(1):87-96
Plant-derivedhumancollagenscaffoldsforskintissueengineering
Willardetal.;TissueEngPartA.2013Jul;19(13-14):1507-18
LipopeptidebiosurfactantpseudofactinIIinducedapoptosisofmelanomaA375cellsbyspecificinteractionwiththeplasmamembrane
Janeketal.;PLoSONE.2013;8(3):e57991
InfliximabinducesdownregulationoftheIL-12/IL-23axisin6-sulfo-LacNac(slan)1dendriticcellsandmacrophages
Brunneretal.;JAllergyClinImmunol.2013Nov;132(5):1184-1193.e8
HeparinincreasestheinfectivityofhumanPapillomavirustype16independentofcellsurfaceproteoglycansandinducesL1epitopeexposure
Cerqueiraetal.;CellMicrobiol.2013Nov;15(11):1818-36
Folliculardermalpapillastructuresbyorganizationofepithelialandmesenchymalcellsininterfacialpolyelectrolytecomplexfibers
Limetal.;Biomaterials.2013Sep;34(29):7064-72
AmygdalinanaloguesinhibitIFN-gammasignallingandreducetheinflammatoryresponseinhumanepidermalkeratinocytes
Paolettietal.;Inflammation.2013Dec;36(6):1316-26
ApurifiedFeverfewextractprotectsfromoxidativedamagebyinducingDNArepairinskincellsviaaPI3-kinase-dependentNrf2/AREpathway
Rodriguezetal.;JDermatolSci.2013Dec;72(3):304-10
ThetranscriptionfactorsTBX2andTBX3interactwithhumanPapillomavirus16(HPV16)L2andrepressthelongcontrolregionofHPVs
Schneideretal.;JVirol.2013Apr;87(8):4461-74
Vinblastine-inducedapoptosisofmelanomacellsismediatedbyRashomologousAprotein(RhoA)viamitochondrialandnon-mitochondrial-dependentmechanisms
Selimovicetal.;Apoptosis.2013Aug;18(8):980-97
IL-17AandIFN-gammasynergisticallyinduceRNase7expressionviaSTAT3inprimarykeratinocytes
Simanskietal.;PLoSOne.2013;8(3):e59531
RetargetingofratparvovirusH-1PVtocancercellsthroughgeneticengineeringoftheviralcapsid
Allaumeetal.;JVirol.2012Apr;86(7):3452-65
ResistancetoHSV-1infectionintheepitheliumresideswiththenovelinnatesensor,IFI-16
Conradyetal.;MucosalImmunol.2012Mar;5(2):173-83
Xenobioticmetabolismcapacitiesofhumanskinincomparisontoa3D-epidermismodelandkeratinocyte-basedcellcultureasinvitroalternativesforchemicaltesting:phaseIIenzymes
Götzetal.;ExpDermatol.2012May;21(5):364-9
Humankeratinocytes\"responsetoinjuryupregulatesCCL20andothergeneslinkinginnateandadaptiveimmunity
Kennedy-Crispinetal.;JInvestDermatol.2012Jan;132(1):105-13
Combinationofsulindacanddichloroacetatekillscancercellsviaoxidativedamage
Ayyanathanetal.;PLoSOne.2012;7(7):e39949
Highthrombinconcentrationsinfibrinsealantsinduceapoptosisinhumankeratinocytes
Gugerelletal.;JBiomedMaterResA.2012May;100(5):1239-47
Asubunitofeukaryotictranslationinitiationfactor2alpha-phosphatase(CreP/PPP1R15B),regulatesmembranetraffic
Kloftetal.;JBiolChem.2012Oct12;287(42):35299-317
EstrADIolprotectsthedermalhyaluronan/versicanmatrixduringphotoagingbyreleaseofepidermalgrowthfactorfromkeratinocytes
Rocketal.;JBiolChem.2012Jun8;287(24):20056-69
Keratinocytegrowthfactorinducesgeneexpressionsignatureassociatedwithsuppressionofmalignantphenotypeofcutaneoussquamouscarcinomacells
Torisevaetal.;PLoSOne.2012;7(3):e33041
MetalallergensnickelandcobaltfacilitateTLR4homodimerizationindependentlyofMD2
Raghavanetal.;EMBORep.2012Nov30;13(12):1109-15
CentrosomallocalizationofthePsoriasiscandidategeneproduct,CCHCR1,supportsaroleincytoskeletalorganization
Tervaniemietal.;PLoSOne.2012;7(11):e49920
AntialarmineffectofticksalivaduringthetransmissionofLymedisease
Marchaletal.;InfectImmun.2011Feb;79(2):774-85
Decisiveroleoftumornecrosisfactor-αforspongiosisformationinacuteeczematousdermatitis
Kerstanetal.;ArchDermatolRes.2011Nov;303(9):651-8
Activationanddifferentiationofmesenchymalstemcells
MishraandBanerjee;MethodsMolBiol.2011;717:245-53
DifferentialmethylationoftheHPV16upstreamregulatoryregionduringepithelialdifferentiationandneoplastictransformation
VinokurovaandvonKNEBelDoeberitz;PLoSOne.2011;6(9):e24451
Anewamyloidosiscausedbyfibrillaraggregatesofmutatedcorneodesmosin
Caubetetal.;FASEBJ.2010Sep;24(9):3416-26
Nicotinicacid-andmonomethylfumarate-inducedflushinginvolvesGPR109AexpressedbykeratinocytesandCOX-2-dependentprostanoidformationinmice
Hansonetal.;JClinInvest.2010Aug2;120(8):2910-9